![]() ![]() Here, we report the development of the new circularly permuted GFP (cpGFP)-based cAMP indicator G-Flamp1, which exhibits a large fluorescence increase (a maximum Δ F/ F 0 of 1100% in HEK293T cells), decent brightness, appropriate affinity (a K d of 2.17 μM) and fast response kinetics (an association and dissociation half-time of 0.20 and 0.087 s, respectively). However, the spatiotemporal dynamics of intracellular cAMP in intact organisms are largely unknown due to low sensitivity and/or brightness of current genetically encoded fluorescent cAMP indicators. Nature Communications volume 13, Article number: 5363 ( 2022)ĬAMP is a key second messenger that regulates diverse cellular functions including neural plasticity. A high-performance genetically encoded fluorescent indicator for in vivo cAMP imaging ![]()
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